Molecular Genetic Epidemiology: A Laboratory Perspective by Ian N.M. Day (Englis

Description: Molecular Genetic Epidemiology by Ian N.M. Day This volume describes high-throughput approaches to a series of robust, established methodologies in molecular genetic studies of population samples. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This volume describes high-throughput approaches to a series of robust, established methodologies in molecular genetic studies of population samples. Such developments have been essential not only to linkage and association studies of single-gene and complex traits in humans, animals and plants, but also to the characterisation of clone banks, for example in mapping of genomes. Chapters have been written by developers or highly experienced end-users concerned with a diverse array of biological applications. The book should appeal to any researcher for whom costs and throughput in their genetics laboratory have become an issue. Notes Principles and practice of molecular techniques used in EpidemiologySpecial emphasis on high-throughput technologies Table of Contents 1 Mapping Genes for Common Diseases: Statistical Planning, Power, Efficiency and Informatics.- 1.1 Introduction.- 1.2 Power and Sampling Considerations.- 1.3 Models of Locus Action.- 1.4 Maximum Likelihood Estimation.- 1.5 Allelic Association.- 1.6 The Malecot Model for Association.- 1.7 Candidate Genes for Allelic Association.- 1.8 Significance Levels.- 1.9 Meta-Analysis.- 1.10 Informatics.- 1.11 Summary.- References.- 2 Human DNA Sampling and Banking.- 2.1 Introduction.- 2.2 A Brief History of DNA Research.- 2.3 Goals and Needs of Human Population Genetics.- 2.4 Selection of the Source Tissue.- 2.5 A Fundamental Change in Sampling Methodology.- 2.6 DNA Isolation and Purification.- 2.7 Sample Storing.- 2.8 Sample Banking.- References.- 3 Microsatellite Genotyping.- 3.1 Introduction.- 3.2 Microsatellite Markers.- 3.3 The Genotyping Process - Experimental Considerations.- 3.4 Data Analysis.- 3.5 Data Management.- 3.6 Hardware.- 3.7 Future Developments.- 3.8 Applications.- References.- 4Minisatellite and Microsatellite DNA Fingerprinting.- 4.1 Introduction.- 4.2 Blood Grouping.- 4.3 DNA Fingerprinting — The Discovery.- 4.4 DNA Fingerprinting — The Applications.- 4.5 An Explosion of DNA Fingerprinting Sequences.- 4.6 Shortcomings of Multilocus Probes.- 4.7 Single Locus Probes — DNA Profiling.- 4.8 Europe and the USA Disagree.- 4.9 Lessons from the Castro Case.- 4.10 Impact of PCR.- 4.11 Digital DNA Typing.- 4.12 Short Tandem Repeat Profiling.- 4.13 The UK National DNA Database.- 4.14 STR Profiling Applications.- 4.15 The Future.- References.- 5 Multiplex Polymerase Chain Reaction and Immobilized Probes: Application to Cardiovascular Disease.- 5.1 Introduction.- 5.2 Multiplex Polymerase Chain Reaction (PCR) Methodology.- 5.3 Sequence-Specific Oligonucleotide Probe (SSOP) Methodology.- 5.4 Example: Candidate Markers of CVD Risk.- 5.5 Summary.- References.- 6 The Special Case of HLA Genes: Detection and Resolution of Multiple Polymorphic Sites in a Single Gene.- 6.1 Introduction.- 6.2 The HLA System.- 6.3 Requirement for HLA Genotyping: Historical Perspectives.- 6.4 PCR-based Approaches to HLA Genotyping.- 6.5 A PCR-SSOP-Based Strategy for HLA Class II Typing Using DNA Derived from Archival Tissue Banks.- 6.6 Concluding Remarks.- References.- 7 Microplate Array Diagonal Gel Electrophoresis (MADGE) Methodologies: The First Five Years.- 7.1 Introduction.- 7.2 Microplate Array Diagonal Gel Electrophoresis (MADGE).- 7.3 CpG-PCR.- 7.4 Temporal Thermal Ramp MADGE Electrophoresis (Melt-MADGE).- 7.5 Software Applications (Applies to All MADGE Not Just Melt-MADGE).- 7.6 Future Developments.- References.- 8 The Use of Sequence Analysis for Homozygote and Heterozygote Base Variation Discovery.- 8.1 Introduction.- 8.2 Sequence Analysis: a Polymorphism Discovery Method.- 8.3 Key Advancements in Sequencing Technologies: Chemistries, Hardware, and Software.- 8.4 Heterozygote and Homozygote Polymorphism Base Calling.- 8.5 Strategies for Sequencing-Based PolymorphismDetection.- 8.6 Summary and Outlook: To Sequence or Not To Sequence — This Is Not in Question.- References. Promotional Springer Book Archives Long Description This volume describes high-throughput approaches to a series of robust, established methodologies in molecular genetic studies of population samples. Such developments have been essential not only to linkage and association studies of single-gene and complex traits in humans, animals and plants, but also to the characterisation of clone banks, for example in mapping of genomes. Chapters have been written by developers or highly experienced end-users concerned with a diverse array of biological applications. The book should appeal to any researcher for whom costs and throughput in their genetics laboratory have become an issue. Feature Principles and practice of molecular techniques used in Epidemiology Special emphasis on high-throughput technologies Details ISBN354041388X Publisher Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Series Principles and Practice Year 2001 ISBN-10 354041388X ISBN-13 9783540413882 Format Hardcover Imprint Springer-Verlag Berlin and Heidelberg GmbH & Co. K Subtitle A Laboratory Perspective Place of Publication Berlin Country of Publication Germany DEWEY 572.8 Publication Date 2001-10-23 Short Title MOLECULAR GENETIC EPIDEMIOLOGY Language English Media Book Pages 214 DOI 10.1007/b75353 Edited by Ian N.M. Day Author Ian N.M. Day Edition Description 2002 ed. Edition 2002nd Alternative 9783540413875 Audience Undergraduate Illustrations 1 Illustrations, color; 204 Illustrations, black and white; XII, 214 p. 205 illus., 1 illus. in color. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96308969;

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